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swing out rotor sw41ti  (Beckman Coulter)


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    Beckman Coulter swing out rotor sw41ti
    Swing Out Rotor Sw41ti, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 96/100, based on 11971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sw41ti+swing+out+rotor/SW+41+Ti+Rotor/pm32294595-92-34-40
    Average 96 stars, based on 11971 article reviews
    swing out rotor sw41ti - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Gradient Centrifugation:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.

    Expressing:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.

    Centrifugation:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.

    Derivative Assay:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.

    Negative Control:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.

    Software:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.

    Purification:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.

    Incubation:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.

    Magnetic Beads:

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures
    Article Snippet: To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.To ensure accuracy, 2 separate sets of DensityMarkerBeads (Amersham Pharmacia Biotech, Uppsala, Sweden) were loaded in 2 separate tubes and centrifuged alongside.. The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).. Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.Following centrifugation, aliquots (0.5 ml) were sequentially removed from the top of the tube, ensuring that the formed gradient was not disrupted.

    Article Title: Intestinal peptidases form functional complexes with the neutral amino acid transporter B 0 AT1
    Article Snippet: The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.The complete membrane extract was then diluted with an equivalent volume of 80% (w/v) sucrose dissolved in the same buffer to create a 40% (w/v) sucrose gradient bed.. The mixture was carefully overlaid with a 5–30% (w/v) linear sucrose gradient and then centrifuged at 3°C at 212000 g (68000 rev./min) for 24 h in a Beckmann SW41Ti swing-out rotor (Beckmann-Coulter).. Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.Sucrose gradient fractions were collected from the bottom of the centrifugation tube in 0.8 ml fractions.



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    Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a <t>SW41Ti</t> rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.
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    Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a SW41Ti rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.

    Journal: Journal of Extracellular Vesicles

    Article Title: Characterization of physical properties of tissue factor–containing microvesicles and a comparison of ultracentrifuge-based recovery procedures

    doi: 10.3402/jev.v3.23592

    Figure Lengend Snippet: Nanoparticle tracking analysis (NTA) of TF-containing microvesicles (1.03–1.08 g/ml) separated by density gradient centrifugation. Microvesicles were prepared from normal human plasma (A), conditioned media from MDA-MB-231 cells (B), and conditioned media from MDA-MB-231 cells expressing TF-tGFP protein (C). The plasma and conditioned media were collected and cleared of any cell debris by centrifuging at 5,400 g on a microcentrifuge, and microvesicles sedimented at 100,000 g . The microvesicles were resuspended in PBS and were fractionated by density gradient ultracentrifugation using a sucrose–OptiPrep gradient covering an approximate density range of 1.02–1.22 g/ml alongside 2 sets of DensityMarkerBeads. The samples were centrifuged at 52,000 g for 90 min at 20°C in a SW41Ti rotor on a Beckman L8-M ultracentrifuge. Following centrifugation, aliquots (0.5 ml) were sequentially removed and assessed for TF antigen. Samples containing TF antigen were then pooled (1.03–1.08 g/ml) and diluted 1:10 in PBS, and the size of the microvesicle population was analysed by NTA using a NanoSight LM10 instrument. A control sample was prepared by adding MDA-MB-231-derived microvesicles to the same pooled fractions from a blank density gradient centrifugation (D). A negative control made of the pooled fractions from a blank density gradient centrifugation showed no detectable trace (E). The illustrations are typical (n=3) of the size distributions which were determined using NTA software. The total amounts of microvesicles in the samples are not comparable. TF-containing microvesicles were immuno-purified from conditioned media of MDA-MB-231 (F) and A375 cell lines (G). The samples were incubated with a monoclonal antibody against TF (10H10; 4 µg/ml) followed by protein A-magnetic beads. The samples were washed with PBS and eluted in phosphate buffer containing NaCl (500 mM). The samples were analysed by NTA against a sample treated similarly but without the antibody.

    Article Snippet: The samples and markers were placed in a SW41Ti swing-out rotor and centrifuged at 52,000 g for 90 min at 20°C on a Beckman L8-M ultracentrifuge (Beckman Coulter).

    Techniques: Gradient Centrifugation, Expressing, Centrifugation, Derivative Assay, Negative Control, Software, Purification, Incubation, Magnetic Beads